Cell Biology
Read more about Faster and Easier Flow Cytometry Sample Prep, Synthetic mRNA for Research and Clinical Labs, Next Generation Cell Recovery for Immuno-Oncology etc.
Read more about Faster and Easier Flow Cytometry Sample Prep, Synthetic mRNA for Research and Clinical Labs, Next Generation Cell Recovery for Immuno-Oncology etc.
Powered by scientifically established Ghost Cytometry technology and three fluorescence lasers, VisionSort enables researchers to perform real-time, AI-driven, single cell sorting with or without labels. Flexible, easy-to-use, and usercontrolled AI algorithms embedded directly in the instrument enable unbiased morphometric characterization and isolation of rare and unique cell populations.
VisionSort core technology Ghost Cytometry was introduced to allow machine vision-based sorting in 4 dimensions.
Applications are cell therapy research and drug discovery.
“Next-generation Sample Preparation for Next-generation Applications” explores the novel and automated Laminar Wash™ system, the only suspension-cell sample preparation workstation designed to eliminate the upstream in-process variabilities caused by the traditional sample preparation method, including labor intensive and hands-on workflows, inconsistencies across users and locations, difficult and time-consuming technology operations, and centrifuge-based mechanical stress on the samples.
Readers will discover the benefits of the Laminar Wash™ system, including increased efficiency, reproducibility, and workflow optimization. The eBook explores the gentle Laminar Wash™ technology and the advanced user-friendly software of the system that automate tedious and error-prone manual sample
preparation steps.
In addition, the eBook discusses the broader implications of this technology for scientific research and analysis in a variety of fields (cell and gene therapy, single-cell multiomics, biomarker discovery, and tumor microenvironment, to name a few). It showcases real-world case studies and success stories, highlighting how automating sample preparation workflows with the Laminar Wash™ system
revolutionizes data quality, throughput, and experimental precision.
Cell-type specific nuclei populations can be used for a wide range of downstream sequencing applications including transcriptomic and epigenomic analyses. Cerevance´s breakthrough use of Laminar Wash™ Technology in their proprietary NETSseq platform has enabled an improvement in nuclei retention as well as reagent cost reductions and time savings.
This Labroots Webinar features David Cadwalladr (Cerevance) and Sashigala Ponnalagu (Curiox Biosystems), and aims to describe a popular workflow that profiles specific brain cell types in healthy and diseased post-mortem brain tissues.
Scientists from Charles River Laboratories and Curiox Biosystems collaborated to characterize the performance and workflow of Laminar WashTM systems in isolating and preparing tumor infiltrating leukocytes (TILs) from anti-PD1-challenged CT26 mice, as a model system.
Compared to conventional centrifuge-based methods, Laminar Wash technology:
The novel Laminar Wash system represents an elegant, reproducible, and partially automated approach to recovering viable and well-defined TIL subpopulations for quantification and analysis by multicolor flow cytometry.
Use this link (or click on the image above) to download a copy of the white paper.
The first and only fully automated sample preparation system designed to enable staining and washing
of suspension cells with minimal hands-on time providing reliability, flexibility and consistency to ensure reproducibility in your lab.
The gentle Laminar Wash™ Technology is integrated with the fully automated Hamilton Nimbus Robotic to dispense, fix/permeabilize, stain, wash, and transfer samples for downstream analysis. The intuitive and easy-to-use software interface comes preprogramed with modifiable protocols, automation novices and experts alike can easily customize workflows using the intuitive user interface.
OZ Biosciences offers mRNAs that mimic fully processed mature mRNAs. These mRNAs are stabilized with 5’ Cap 1 structure and 3’ poly(A) tail and are optimized to yield improved stability & performance. They are modified with 5-methoxyuridine (5moU replaces U) or N1-methyl-pseudouridine (N1-mψ) to reduce innate immune responses. We also offer unmodified mRNAs.
5 categories of ready-to-use mRNAs :
Reporter gene mRNA´s: ideal controls to study transfection efficiency
Genome editing mRNA´s: Cas9 mRNA for CRISPR Genome editing
Vaccine mRNA´s: ideal as controls for immunization or vaccine studies
Gene replacement mRNA´s: EPO mRNA
Fluorescent mRNA´s: Cy5 GFP mRNA, Cy5 GFP mRNA
Automated, consistent, and standardized cell preparation solution
Despite their limitations and intensive hands-on requirements, centrifuges have been the only solution to washing cell suspension prior to analysis.
Now with Laminar Wash Systems, you can improve your workflow, increase cell retention and generate more robust, reproducible data.
Challenges of centrifugation washing:
Pellets debris along with cells
Requires several manual steps prone to variation
Requires flinging or aspirating supernatants, which impacts reproducibility
Reduces cell retention and viability
Methods
Efficient and thorough cell washing is a necessary but labor-intensive process in sample preparation for cytometry analysis. The Laminar Wash AUTO1000 system completely automates the staining and washing workflow, eliminating a major source of variability and enabling process standardization. Designed with the scientist in mind, the AUTO1000 offers several key benefits to research and shared resource laboratories in cell and gene therapy discovery and process development, antibody screening, flow core labs, and other applications that rely on cell-based assays, including:
Save time and effort with Laminar Wash technology in preparation of suspension cells.
Total run time and hands-on time are reduced, while cell retention is increased.
The collaboration will support standardization efforts in live cell analysis to improve and accelerate the development of next-generation therapies, vaccines, and biomanufacturing.
According to NIST, the Flow Cytometry Standards Consortium was launched in December 2020 “to accelerate the adoption of quantitative flow cytometry in biomanufacturing of cell and gene therapies.” Technical Lead and NIST Senior Scientist Lili Wang, Ph.D. described the consortium’s mission in a recent Photonics article as, “[providing] a neutral forum for stakeholders in the biotechnology and health care sectors to identify and address common measurement challenges, exchange ideas, and jointly accelerate the development of standards and reference materials for quantitative flow cytometry.”
In support of the Consortium’s mission, Curiox will contribute its expertise and fully turnkey sample prep automation solution, the Laminar Wash AUTO1000 system to facilitate the adoption of universal flow cytometry methodology. Additionally, Curiox Vice President David Choiniere will serve on the Consortium Steering Committee overseeing research activities and progress, with the goal of publishing collective results.
As part of the Consortium, Curiox hopes to address challenges in flow cytometry faced by the biomanufacturing community, notably reproducibility, comparability, and accurate quantitation. Curiox and NIST scientists will collaboratively develop automated sample preparation protocols to characterize common immunotherapy cell types, as well as utilize critical reagents and additional measurement capabilities beyond flow cytometry.
The NEW super compact and intuitive live cell imaging system, Celloger Nano – got everything you need to perform your sophisticate laboratory works. Equipped with exceptional fluorescence and auto-focusing technology, precise stage controller, and user-friendly software, accelerates your cell-based research works. Expand your cell-based research through our multifunction live cell imaging system, Celloger Nano.
RNA Therapeutics os booming and the mRNA field is generating huge expectations.
The synthetic, high quality mRNA from OZ Bioscicences are available and ancoding both as off-the shelf genes as well as design for custom genes.
Reporter gene mRNAs: ideal controls to study transfection efficiency
Genome editing mRNAs: Cas9 mRNA for CRISPR genome editing
Vaccine/Antigen mRNAs: optimal as controls for immunization or vaccine studies
In a recent collaboration between Cerevance in Cambridge and Curiox Biosystems, brain nuclei were processed for single cell sequencing.
Benefits of using Laminar Wash were:
Laminar Wash technology replaces centrifugation based wash of cell suspensions for flow cytometry, mass cytometry and single cell genomics/proteogenomics.
Watch this time motion study video to see how
Scientists from Charles River Laboratories and Curiox Biosystems collaborated to characterize the performance and workflow of Laminar Wash™ systems in isolating and preparing tumor infiltrating leukocytes (TILs) from anti-PD1-challenged CT26 mice, as a model system.
Compared to conventional centrifuge-based methods, Laminar Wash technology:
Successfully executing a 43 color panel is a technically demanding task. One that is made more difficult when there are confounding issues like non-specific binding and cell debris.
The methods described in this new publication in Cytometry Part A show that the preparation of the samples was very similar to traditional cell staining with the exception that Laminar Wash Technology was used to prepare the cells in addition to the large number of antibody types used. Because the results that were presented showed incredibly high reproducibility, including at low cell numbers, it would seem safe to posit that preparing cells with Laminar Wash technology is key in providing quality reproducible data.
siPOOLs™: Complex siRNA Pools for Specific and Reliable RNA Interference
siPOOLs are defined, high complexity pools of 30 siRNAs designed for maximal transcript coverage.
Avoid off-target effects
Reliable phenotypes
Efficient knockdown (at low nM)
Guaranteed performance
Efficient siRNA transfection of primary cells and cell lines
SilenceMag siRNA Delivery Reagent is based on the Magnetofection™ technology. Simple, rapid and easy to use, SilenceMag is serum compatible and non toxic. Specifically designed for siRNA transfection, SilenceMag gives reliable high protein knockdown at very low doses of siRNA in numerous cell types (primary cells, hard-to-transfect & cell lines).
Lullaby is the ideal siRNA transfection reagent for gene silencing. Relying on the TEE-technology, it has been successfully tested on numerous cell lines, reaching up to 90% gene silencing with high reproducibility and a very low toxicity. RNA interference is a powerful technique to shut down genes expression in cells and organisms.
ViroMag Stem, a Magnetofection-based Lentiviral Transduction Enhancer, enables improved viral driven genetic modification in a wide range of stem cells in different applications, such as ex vivo gene therapy and cell therapy.
ViroMag Stem, as a stabilized magnetic nanoparticles formulation, offers a simple and reproducible method for increasing lentiviral infection and transduction of difficult cell types such as CD34+ hematopoietic stem cells, both cell lines and primary cells, in cell culture plate using the Magnetofection technology.
This method combines maximum cell viability and high transduction efficiency while cell phenotype and differentiation potential are not affected.
ViroMag Stem outperforms lentiviral mediated transduction efficiency in CD34+ cell lines
KG1a CD34+ cells were infected at MOI 5 (5 viral particles per cell) using a HIV-SFFV-GFP lentivirus in presence of viral enhancers at the recommend doses. 72 hours after transduction, % of GFP positive cells (A) and total intensity (B) were analyzed by flow cytometry.
Faster and automatible, Laminar Wash™ systems deliver higher quality data through better cell retention, better preservation of cellular physiology and viability, and improved study-to-study and operator-to-operator reproducibility.
We now offer mRNAs that mimic fully processed mature mRNAs.
These mRNAs are stabilized with 5’ Cap 1 structure and 3’ poly(A) tail and are optimized to yield improved stability and performance.
They are modified with 5-methoxyuridine (5moU replaces U) to reduce innate immune responses. We also offer unmodified mRNAs.
Reporter gene mRNAs: ideal controls to study transfection efficiency
Genome editing mRNA: Cas9 mRNA for CRISPR Genome editing
Vaccine mRNAs: ideal as controls for immunization or vaccine studies
Lullaby Stem siRNA Transfection Reagent is ideal for gene silencing in Stem Cells. Lullaby Stem enables siRNA and miRNA transfection of multipotent and embryonic stem cells with high efficiency and very low toxicity. Its lipid-specific formulation protects small RNA from extracellular degradation, transports them across cell membranes and efficiently releases them into stem cell cytoplasm according to the TEE-technology.
The new VaxOZ vaccine adjuvants from OZ Bioscience are perfect for any immunization experiment.
Currently, adjuvants remain to be the most important strategy to improve the efficiency of conventional and next-generation vaccines. Genetic adjuvant is an immunological agent uses in combination with a specific antigen in order to boost the immune response. The direct consequence is to produce more antibodies and longer-lasting immunity than with the antigen alone and thus minimizing the dose of antigen needed to the vaccine. In particular, DOTAP (1,2-dioleoyl-3-trimethylammonium-propane) based cationic lipids induce maturation of dentritic cells, which then traffic to the draining lymph nodes to activate antigen-specific T cells.
In vivo grade manufactured and quality controlled, the VaxOZ adjuvants are intended for research purposes only.
CaLiVax DOTAP cationic lipid-based composition for liposome-mediated DNA or protein vaccines.
PolyVax-CPO Vaccine Adjuvant is a cationic polymer genetic adjuvant that associates with plasmid DNA to form an efficient polymer-based nanoparticle delivery system (NPD).
CNE-CPO (Cationic Nano Emulsion) Vaccine Adjuvant is an oil-in-water Cationic Nano Emulsion made of squalene droplets and cationic polymers in a continuous aqueous phase. CNE-CPO Vaccine Adjuvant is biodegradable, an important advantage over alternative oils that have been used in emulsion adjuvants.
Single-cell genomics and proteogenomics workflows can be greatly improved by the Laminar Wash™ technology.
The Laminar Mini is the perfect tool for single-cell sequencing experiments. It washes 8 samples simultaneously in a few minutes. It replaces centrifugation and preserves precious samples at low cell numbers. The workflow is easier and faster.
In the recent webinar, Curiox, Biolegend and ImmunAI presented data on increased cell retention compared to centrifugation, higher signal-to-noise ratios and time savings.
Celloger Mini is a compact and easy-to-use live cell imaging system.
The Lentiblast Premium Transduction Enhancer is designed to enhance viral transduction in many types of primary cells – adherent and suspension, especially also in CD34+ hematopoietic stem cells or T-lymphocytes. The image shows data on the effect of using LentiBlast Premium in CD34+ stem cells at a low viral particle/cell number ratio.
LentiBlast Premium is a patented reagent from OZ Biosciences with the following key benefits:
Magnetic Assisted Transfection is a novel technology developed by OZ Biosciences. It has a proven high efficiency in delivering molecules into challenging cells. Magnetic nanoparticles are associated with nucleic acids (naked or pre-complexed with a transfectionreagent or viruses) by salt-induced aggregation and electrostatic interactions.
Magnetic force drives these complexes towards the target cells, allowing a rapid concentration of the vector dose onto cells
The cellular uptake of the genetic materials is accomplished by endocytosis and pinocytosis
Nucleic acids are released in the cytoplasm by flip-flop mechanism or proton sponge effect*
* Plank et al , Adv. Drug Deliv. Rev. (2011), 63(14-15):1300-31
The reagents from OZ Biosciences covers a large range of applications:
Single-cell genomics is rapidly growing. Nature Methods named multimodal omics the method of the year for 2019. And as more scientists use single-cell techniques, they find that sample preparation is very important to achieve high-quality data.
Single cell sequencing can suffer from poor data quality due to clumping of cells, incomplete washing of cells leaving cell-free nucleic acids, and poor retention of low numbers of cells. Washing via centrifugation causes stress on the cells in samples and can cause erroneous gene expression results.
Sample prep is extremely important in single-cell genomics, especially for single cell gene expression and immune profiling. In this area, mRNA transcripts are being barcoded and cells can experience mRNA leakage (causing increased background) or mRNA degradation (causing less signal). Unlike with flow cytometry, in single-cell sequencing it is not possible to gate out debris or groups of cells. The cell preparation must be as clean as possible and consist of single cells.
By using the Laminar Wash system scientists can see time savings and less manual manipulation.
Laminar Wash systems can wash cells without putting stress on cells that may impact gene expression, cell integrity and viability.
Nuclei Isolation Using Laminar Wash
PBMCs were lysed in bulk in tube with Lysis Buffer, quenched with Wash Buffer and centrifuged
Cells are further either washed twiced in tube by centrifuge, OR plated in Laminar Wash plate to settle for 20min, washed 7x in a Laminar Wash HT1000 System with PBS + 0.04% BSA
Reliable, Automated and Reproducible Flow Cytometry
The unique Laminar Wash™ technology simplifies and automates flow cytometry workflows for CAR-T-cell manufacturing. Data improves reproducibility, saves time and gives tighter populations. Laminar Wash™ replaces centrifugation-based stain&wash and is more gentle to cells. Time saving in this workflow was 50 min/assay.
CAR-T cell therapy biotech company AdiCet Bio improved data by automating of a part of their flow cytometry assay. They saw improved consistency and are planning full automation using a Laminar Wash™ system.
The DA-Cell Laminar wash technology replaces centrifugation by using a novel DA-Cell technology, which is gentle to cells, giving higher retention.
siPOOLs™ are innovative, complex siRNA pools which reduce false positives & false negatives in siRNA knockdown experiments
Individual siRNAs or low complexity siRNA pools containing 3-4 siRNAs often hit multiple off-target genes and exhibit variable target gene knock-down. Short interfering RNA (siRNA) pools, siPOOLs are highly complex and defined pools of 30 siRNAs, each siRNA present at picomolar working concentrations.
Benefits:
Download the new TechNote:
Off-Target Effects; Causes, Extent & Impact
Publication:
Hannus et al NAR
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