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Multiplexing 101: Unlocking Critical Single-Cell Insights with Increased Throughput and Efficiency

Thursday June 6 2024, 5:00 pm (CET)

Despite the richer insights that single-cell multi-omics can offer over conventional techniques such as PCR and bulk next-generation sequencing, including characterizing the clonality of heterogeneous cancer cells and changes driving therapy resistance or assessing genome editing outcomes, the costs for sample analysis can be a barrier to adoption for many labs.

To help address this, Mission Bio has recently launched new sample multiplexing features that allow the pooling of multiple samples into a single run on the Tapestri Platform, improving the throughput and productivity, and thereby reducing the per-sample costs for single-cell DNA and protein multi-omic analysis by up to 60%*.

In this educational webinar, we’ll discuss two novel multiplexing approaches available on Tapestri, Antibody Hashing and Genotype Multiplexing. We’ll explore how they enable increased efficiency and versatility while maintaining the same robust performance metrics such as sensitivity and specificity. We’ll also share real-world case studies from labs that are applying multiplexing approaches in disease modeling, CRISPR-editing, and hemato-oncology applications.

EMERGE Episode 19 with Sudip Ghosh “Deep proteomic insight into disease biology identifies age-specific vulnerabilities in acute leukemia”

Wednesday May 29 2024, 4:00 pm (CET)

Infant and adult MLL1/KMT2A-rearranged (MLLr) leukemia represents a disease with a dismal prognosis. Here, we characterized early proteomic events of MLL::ENL-mediated transformation in fetal and adult blood progenitors and reveal that whereas adult pre-leukemic cells are mainly characterized by retained myeloid features and downregulation of ribosomal and metabolic proteins, expression of MLL::ENL in fetal lymphomyeloid multipotent progenitors (LMPPs) leads to enrichment of translation-associated and histone deacetylases signaling proteins, and decreased expression of inflammation and myeloid differentiation proteins. Integrating the proteome of pre-leukemic cells with their secretome and the proteomic composition of the extracellular environment of normal progenitors highlights ligand-receptor interactions with differential regulation upon initiation of fetal- and adult-origin leukemia. We show that these interactions have implications for human MLLr leukemia cells’ ability to communicate with their environment through granule proteins. Our study has uncovered opportunities for targeting ontogeny-specific proteomic vulnerabilities in in utero-initiated and adult-onset MLLr leukemia.

EMERGE Episode 18 with Patricia Bortel and “Systematic optimization of automated phosphopeptide enrichment for high-sensitivity phosphoproteomics”

Wednesday April 24 2024, 4:00 pm (CET)

Improving coverage, robustness and sensitivity is crucial for routine phosphoproteomics analysis by single-shot liquid chromatography tandem mass spectrometry (LC-MS/MS) from minimal peptide inputs. The presentation focuses on our systematic optimization of key experimental parameters for automated on-beads phosphoproteomics sample preparation with focus on low input samples, pinpointing critical variables influencing the resulting phosphoproteome. Furthermore, we evaluated how sequential enrichment can boost phosphoproteome coverage and show that pooling fractions into a single LC-MS/MS analysis can increase the depth. The presentation also includes an alternative phosphopeptide enrichment strategy based on stepwise addition of beads thereby boosting phosphoproteome coverage. Our optimized phosphoproteomics pipeline can be translated to the newest generation of mass spectrometers, such as the Orbitrap Astral, increasing the phosphopeptide coverage by 2-fold, allowing for deep phosphoproteomics analysis without the need to scale up the starting cell amounts.

Streamlined proteome-wide identification of drug targets indicates organ-specific engagement

Wednesday April 3 2024, 4:00 pm (CET)

Proteins are the primary targets of almost all small molecule drugs. Nonetheless, even drugs designed with high specificity may interact with multiple proteins that are not initially targeted. Discovering these potential interactions is crucial for the development and repurposing of drugs. Current state-of-the-art proteomics methodologies enable screening of thousands of proteins against a limited number of drug molecules. Here we report the development of a label-free quantitative proteomics approach that enables proteome-wide screening of small organic molecules in a scalable, reproducible, and rapid manner by streamlining the proteome integral solubility alteration (PISA) assay.


Immunopeptidomics: Isolation of sHLA Peptides from Plasma

Wednesday Nov 8 2023 5 pm (CET)

Soluble HLA (sHLA) peptides are thought to have great potential as biomarkers for different diseases such as cancer. However, efficient enrichment of sHLA peptides from body fluids like plasma has been a bottleneck, requiring 3-5 ml per patient. Here we present IMBAS-MS, an automated magnetic bead based workflow for the efficient enrichment of HLA protein peptide complexes.

The core of IMBAS-MS is the combination of biotinylated antibodies captured by streptavidin magnetic beads allowing for an automated one pot enrichment from only 200 µl plasma.

In combination with Whisper low-flow gradients, data-independent acquisition (DIA) and computational library-based analysis we acquire deep immunopeptidomes from single measurements, elucidating its origins.

Applied statistics in the Medical Laboratory

Tuesday Oct 31 2023 3 pm (CET)

Our guest speaker for the series will be Stephen MacDonald, CPD assessor and a Principal Clinical Scientist at Cambridge University Hospitals NHS Foundation Trust.

In the first of the three sessions, we will introduce commonly used statistical methods, terms and concepts. Topics will include statistical summaries such as mean, variance, standard deviation and coefficient of variation.

We will draw the distinction between imprecision and bias, and how both contribute to the quality of our results. 

We will learn how statistics are used and what other calculations they feed into, including total error and measurement uncertainty. Using practical examples and case studies to illustrate the concepts discussed we can understand how to apply these statistical principles in your daily laboratory operations.

By the end of this session, participants will:

  1. Gain proficiency in basic statistical measures such as standard deviation and coefficient of variation to quantify and manage analytical variability
  2. Be able to distinguish between systematic and random errors
  3. Acquire the knowledge and skills to calculate total error in laboratory measurements, taking into account both systematic and random error components.
  4. Participants will be introduced to the basics of how statistics are used in understanding Measurement Uncertainty, to combine analytical variability and metrological traceability of laboratory results.

Whether you are a seasoned professional or new to the field, this session will provide valuable insights and practical knowledge that can be immediately applied in your workplace.

About Stephen MacDonald

Stephen MacDonald is a Principal Clinical Scientist in the field of Specialist Hemostasis working at Cambridge University Hospitals NHS Foundation Trust.

Stephen has a keen interest in the statistical assessment of laboratory assay performance and clinical trials. He has focused on determination of uncertainty of measurement in Specialist Hemostasis assays and in developing models to best describe their performance.

This has led Stephen to participating in National Advisory boards for numerous aspects of Laboratory Hemostasis as well as publishing a range of journal articles in The Journal of Laboratory and Precision Medicine and the International Journal of Laboratory Hematology.

Myeloma Matters: Advancing Research and Therapy Development with Single-cell DNA + Protein Multi-Omics

Wednesday Oct 18 2023 5 pm (CEST)

Multiple myeloma is a complex form of plasma cell cancer that is currently untreatable. There are approximately 200,000 new diagnoses each year globally, with a 100% relapse rate and 54% five-year overall survival rate. In spite of significant advancements in treating blood-related cancers, these diseases continue to be a substantial cause of illness and death worldwide.

In this webinar, Dr. Todd Druley, MD, Ph.D., Chief Medical Officer of Mission Bio, and Co-Founder and Chief Technology Officer Dr. Adam Sciambi, Ph.D., will:

• Discuss the clinical impact and horizon of multiple myeloma therapies

• Present a novel approach to multiple myeloma therapeutic development by correlating SNVs, CNVs, and surface epitopes for clonal profiling

• Share clinical data generated by researchers from The Cancer Research Center of Toulouse IUCT-Oncopole and Genentech

Webinar: Pre-Eclampsia and Improving Clinical Pathways with PlGf / sFlt-1

Tuesday Oct 10 2023 3 pm (CEST)

Quantitative determination of placental growth factor (PlGF) and soluble fms-like tyrosine kinase-1 (sFlt-1) in human serum and plasma

This webinar will focus on Pre-Eclampsia and improving clinical pathways with PkGF / sFlt-1, including a brief introduction of Pre-eclampsia and insight into the inclusion of biomarkers sFlt-1 & PlGF.

We will be joined by Professor Tim James, Laboratory Manager and Head BioMedical Scientist in Clinical BioChemistry at Oxford University Hospitals.

Professor James will be discussing his own experiences from Oxford to provide insight, advice and guidance to highlight the clinical significance and favourable outcomes of sFlt-1 & PlGF.

About Professor Tim James:

Professor Tim James is Laboratory Manager and Head Biomedical Scientist in Clinical Biochemistry at Oxford University Hospitals NHS Foundation Trust, a post he has held for over 20 years.
Within this role he manages three laboratory sites including a large teaching hospital service. Working with clinical departments to better understand testing and to ensure optimal utilisation is a key aim of the service. He has worked with many clinical teams to evaluate current testing and where possible introduce new diagnostics where there are clear benefits for patients.
He has published over 120 peer reviewed papers in all areas of clinical chemistry and in the last ten years developed a strong collaboration with the Oxford Obstetric team which has led to a series of publications encompassing diagnosis of pre-eclampsia, developing pregnancy specific reference intervals and identifying newer markers of obstetric disease.


Webinar: Quality Control in Laboratory Diagnostics - Practical Guidance in Troubleshooing

Tuesday June 13 2023 4 pm (CEST)

Join us at Randox Webinar: Quality Control in Laboratory Diagnostics – Practical Guidance in Troubleshooting.

We will be hosting the second webinar in our series with Dr Mairiead MacLennan on 13th June 2023 at 3PM (BST). 

Dr Mairiead MacLennan manages a diagnostic laboratory in the United Kingdom with over over 40 years multidisciplinary experience, focused on Quality and Training and will be discussing her own experiences to provide insight, advice and guidance

The second webinar will focus on:

  • Commutability – dealing with lot-to-lot changes
  • Performance Trending – making decisions when there are problems
  • Daily Actions & Monthly Reporting

Dr Mairiead MacLennan is a registered HCPC Biomedical Scientist with over 40 years multidisciplinary diagnostic laboratory experience in both NHS and Veterinary medicine specialising in Quality and Training.

  • Chair of the IBMS Scotland Training Forum
  • Vice Chair of the of the IBMS Scottish Quality Management Discussion Group
  • Member of the Scottish Microbiology and Virology Network
  • Technical Assessor for UKAS


If you cannot attend, please fill out the registration form and we will send you a full recording post webinar!

Webinar:Development and optimisation of a high-throughput proteomics workflow for biomarker discovery in clinical cohorts

Thursday Mar 30, 2023 05:00 PM (CEST)

Breast cancer is the most diagnosed cancer in the world. Clinically, receptor status and lymph node involvement are essential to stratify patients in different treatment groups. Moreover, molecular subtyping of breast tumours further assists in providing information about prognosis and treatment response. However, complications such as overtreatment, overdiagnosis and late recurrence are still a reality. Therefore, improvements in personalised medicine for better stratification and tailoring of treatment are a necessity. Since proteins are more closely related to phenotype, and information such as post-translational modifications affect protein activity, the use of proteins as biomarkers is of interest. In label-free mass spectrometry (MS)-based proteomics, different acquisition strategies can be used, namely data dependent acquisition (DDA) and data independent acquisition (DIA). Here, we establish a new semi-automated bead-based workflow for high-throughput processing of clinical samples, and further evaluate different acquisition methods. We then investigate the potential use of proteome data in comparison with already available matched transcriptome data in the context of personalised medicine and biomarker discovery.

Webinar: scTAM-seq: A Novel Method for Profiling DNA Methylation in Single Cells

Thursday Feb 23, 2023 11:00 AM in Eastern Time (US and Canada)

Genome-wide bulk sequencing is widely used to assess DNA methylation (DNAm). However, methylation signals obtained from bulk sequencing are averaged out, concealing the cell-to-cell differences in methylation signatures involved in health and disease. Single-cell methods such as bisulfite sequencing were developed to reveal these signatures and offer insights into cellular heterogeneity and rare cell types. However, genome-wide DNAm profiling at single-cell resolution has drawbacks such as sparse data and limited genomic coverage even for deeply sequenced samples.

To address this gap, the Renée Beekman Lab and the Lars Velten Lab of the Centre for Genomic Regulation (CRG) in Barcelona teamed up to develop a novel high-throughput, high-confidence targeted bisulfite-free method for analyzing DNAm in single cells, now published in Genome Biology.

In this webinar, authors Michael Scherer, Ph.D. and Agostina Bianchi of CRG will present the Single-Cell Targeted Analysis of the Methylome (scTAM-seq) protocol which profiles 650 biologically relevant CpGs in up to 10,000 cells using the Tapestri platform. They will also share how they combined DNAm (epigenetics), somatic mutations (genotype), and surface marker expression (phenotype) to gain a rich picture of clonal architecture and dynamics across B cell differentiation in blood and bone marrow.

Webinar: Critical Quality Metrics in Cell Sample Prep for Cell- and Gene Therapy Bioanalytics Series

Thursday December 15, 2:00 pm ET (8:00 pm CET)

Cell-mediated immunotherapies are an emerging modality that can revolutionize cancer research as well as the study of the immune system. However, establishing standardization and scalability for necessary bioanalytics into product release practices are hampered by two major challenges:

a)      Requirements for patient-specific or wide-sourced starting materials and

b)      Workflow changes when moving from research applications to regulated process developments.

This talk emphasizes the role of partnerships between early- and late-stream analytical assay developers. While tackling unique challenges in validating flow cytometric methods for Chimeric Antigen Receptor (CAR) T cells, during manufacturing and clinical monitoring. Additionally, evolving targets during transferability and scalability also need to be addressed through Control-Design modalities like increasing automation and integration of various processes.

We will be introducing Critical Quality Attributes and key considerations when designing sample preparation methods, optimization, validation, and implementation for cell and gene therapy bioanalytics.

Emerge Webinar Series Episode 12 RNA-Protein interactions in space and time

Tuesday, 29th November, 17:00 CET (11:00 EDT)

The EMERGE webinar series is an open platform for emerging researchers to share their work highlighting novel methods and solutions they are implementing to tackle the challenges associated with applying omics technologies in a routine way. 

The webinar will focus on two multiomics methodologies that probe the crosstalk between the RNA and the protein worlds, with a specific focus on characterising RNA-protein interactions and RNA and protein subcellular localisation.

OOPS (Orthogonal Organic Phase Separation) repurposes standard Trizol RNA and protein extraction to unbiasedly retrieve all RNA binding proteins and protein bound RNA (Queiroz, Smith & Villanueva, Nature Biotechnology 2019Villanueva, Smith & Queiroz, Nature Protocols 2020). The unbiased nature of OOPS allows for application across variety of human cells and the first bacterium RNA-binding proteome was retrieved. More recently, LoRNA (Localisation of RNA) was developed, a new single-step subcellular fractionation technology to quantitatively interrogate, at once, the subcellular localisation of the transcriptome, both in organelles and phase separated compartments. Simultaneous interrogation of all localisations allows the system-wide quantification of RNA proportional distribution and the comprehensive analysis of RNA subcellular dynamics. Importantly, by coupling LoRNA and LOPIT (Localisation Of Proteins by Isotope Tagging), a simultaneous system-wide map of the transcriptome and proteome could be obtained. By applying this combined framework to cells undergoing the Unfolded Protein Response the first dynamic map of how the cell co-ordinately reorganises its transcriptome and proteome in stress conditions was generated with unprecedented resolution (Villanueva, Smith & Pizzinga, BioRxiv 2022.

Eneko studied Biology at the Pompeu Fabra University, and later obtained a PhD in Biomedical Research in the University of Barcelona. His research focused on how viruses evolve to optimise their protein synthesis; as well as learning how to engineer them to constrain this synthesis to tumours and develop oncolytic viruses. Currently, as a Welcome Trust Postdoctoral Fellow in the Cambridge Center for Proteomics, he is following the “Keep It Simple” paradigm to develop new technologies combining transcriptomics and proteomics to understand how RNA and proteins interact in space and time. 

Single-Cell Multi-omics Symposium: AML, CLL, ALL, Epigenetics, Breast Cancer Virtual Event

Thursday Nov 3 11 am CET

Join the Josep Carreras Leukaemia Research Institute (IJC) and Mission Bio for a special in-person symposium where we discuss the tools and treatments shaping the battle to end cancer.

Quantitative characterization of genotype and phenotype at the single-cell level reveals clonal phylogeny and selection at higher resolution, ultimately improving personalized therapeutic selection. By identifying SNVs, CNVs, and surface protein changes and revealing co-occurrence and zygosity of mutations in single cells, Tapestri enables comprehensive insights into disease progression, development of therapy resistance, and MRD.

Our invited speakers will present data from different areas of cancer research like acute myeloid leukemia, chronic lymphocytic leukaemia, myelodysplastic syndrome, acute myeloid leukemia, breast cancer, chronic lymphocytic leukemia, and acute lymphocytic leukemia.

This event is in-person with a virtual attendance option. To register for in-person or virtual attendance and view the full agenda, visit our registration page.


Applying MARS® for Pre-Enrichment of Breast Cancer Disseminated Tumor Cells in Bone Marrow

Webinar Wed Oct 12 at 2 pm EDT

MARS is the platform of choice for CTCs, DTCs and MRDs.  The system offers recovery as low as 1 in 5 million.  The platform preserves rare tumor cells, ensuring these cells are preserved for analysis.  Larger tumor cells can be easily enriched with our acoustics method wash before requiring magnetic enrichment.  There is no need for centrifugation.  Less manipulation means higher recovery and higher viability.  Our small fluidics pathway minimizes the loss of “sticky” cells.

Join us for a webinar introducing MARS® technology and its use examples. Our Guest Elizabeth M. Chislock, Ph.D., is a Senior Research Investigator in the laboratory of Dr. Lewis Chodosh in the Department of Cancer Biology at the University of Pennsylvania. She received her Ph.D. in Molecular Cancer Biology from Duke University in 2013.

Dr. Chislock’s primary research interest is in investigating the biology of disseminated tumor cells in the bone marrow in order to better understand the mechanisms underlying breast cancer dormancy and recurrence, including the application of novel pre-enrichment methodologies for improved detection and isolation of these rare tumor cells.

Single Cell NGS Sample Prep: Impact of Novel Laminar Wash™ on Nuclei Retention and Other Downstream Applications – A Cerevance Story

Wed Sep 21 7:00 pm (CEST)

This talk aims to describe a popular workflow that profiles specific brain cell types in healthy and diseased post-mortem brain tissues.
This extensive procedure consists of tissue homogenization with nuclei extraction, fixation, and antibody staining followed by fluorescence-activated sorting of nuclei. Cell type-specific nuclei populations can be used for a wide range of downstream sequencing applications including transcriptomic and epigenomic analyses.
Cerevance’s breakthrough use of Laminar Wash™ Technology in their proprietary NETSseq platform has enabled an improvement in nuclei retention as well as reagent cost reductions and time savings.

The webinar will be available for unlimited on-demand viewing after live event.

Single Cell NGS Webinar: Expert Advice on Sample Prep Protocols for Single-Cell Biology Research

Mon June 20 at 4 pm CEST (7 am PT)

This webinar will provide a scientist’s perspective on the importance of sample preparation protocols for single-cell biology research.

New single-cell technologies have facilitated deeper exploration of inter- and intratumor heterogeneity than previously possible, but before any downstream analyses, cells need to be processed into suspensions that are free of debris and aggregates.

Preserving original cell biology and eliminating interfering debris as much as possible across applications is crucial to overall data quality and reproducibility. This webinar will discuss automation solutions and other sample prep protocols that will help you ensure better, standardized sample preparation for fluorescence-based assays.

Single-Cell Multi-Omics for Hematologic Malignancies Research

Thursday June 16 2 pm CEST


Join Mission Bio & the Josep Carreras Leukaemia Research Institute for an interactive virtual seminar highlighting the key advantages of single-cell technology that simultaneously measures single-nucleotide variation (SNV), copy number variation (CNV), and protein data from the same cells. Our multi-omics platform connects genotype and phenotype in each cell, dissecting the architecture of tumors with unprecedented resolution.

We are happy to welcome Dr. Manel Esteller, Director of Josep Carreras Leukaemia Research Institute (IJC). He will present data from his institute on epigenome and epi-transcriptome maps of  normal and transformed cells of leukemia and other malignant blood diseases.

In addition, Simone Formisano will give an introduction to Mission Bio’s Tapestri capabilities on SNV, CNV, and protein sequencing at scale, the workflow and its applications in oncology and cell and gene therapy.

Dr. Manel Esteller, Josep Carreras Leukaemia Research Institute (IJC), Barcelona

Simone Formisano
Technical Application Liaison
Mission Bio.

Bringing a New Standard of Clarity to Solid Tumor Research With Single-Cell Genomics

Thursday June 16 11 am EDT

A major challenge to combating cancer is the dynamism and inherent heterogeneity contributing to the disease’s onset, progression, and relapse. In the enormous efforts to identify the factors underlying these factors, bulk sequencing is widely used, but the need for unmasking the underlying genetic diversity across cell populations remains unmet.

Single-cell DNA sequencing can be used to interrogate clonal mosaicism and neoplastic transformation, metastatic dissemination, and therapeutic resistance with its ability to co-analyze SNVs and CNVs at the single-cell level in a scalable manner. As a tool for probing clonal architecture and evolution, researchers can identify zygosity, mutational co-occurrence, and rare mutations in individual cells.

Jorge Reis-Filho of Memorial Sloan Kettering Cancer Center will share the strategies for characterizing intratumor heterogeneity and evolutionary trajectories in cancers at the single-cell level and present his latest findings in breast cancer research.

This webinar will also present the latest advances in single-cell DNA sequencing, including pre-designed solid tumor DNA panels, single-cell CNV analysis workflow, and nuclei isolation protocols enabling solid tumor researchers to access this technology.

Attendees will:

  • Learn how single-cell DNA sequencing can bring a new level of clarity to research in solid tumors, such as breast cancer, by providing insights into the clonal architecture, mutation co-occurrence, and rare mutations driving tumor progression and therapy resistance.
  • Discover the science behind the Tapestri single-cell sequencing platform.
  • Hear about case studies and implementation considerations, including cell and nuclei prep, panel design, and analysis.



Jorge Reis-Filho, MD, PhD, FRCPath, Chief, Experimental Pathology Service Memorial Sloan Kettering Cancer Center

mission bio Unravel the threads

Unleashing the Power of Single-Cell Multi-Modal Analysis to Advance Precision Biology

Tuesday May 10 at 15:00 CET

Join Mission Bio for an interactive virtual seminar highlighting the key advantages of single-cell technology that simultaneously measures single-nucleotide variation (SNV), copy number variation (CNV), and protein data from the same cells. Our multi-omics platform connects genotype and phenotype in each cell, dissecting the architecture of tumors with unprecedented resolution.

We are happy to welcome Dr. Kendell Clement, Instructor in Pathology at the Massachusetts General Hospital who will present at this seminar.

In addition, Simone Formisano will give a short introduction to Mission Bio’s Tapestri capabilities on SNV, CNV, and protein sequencing at scale, the workflow and its applications in oncology and cell and gene therapy.

CHIP’ing away at somatic mosaicism — Diving into the promises and challenges of characterizing clonal hematopoiesis of indeterminate potential

Friday April 22 11:30 am EDT

CHIP (clonal hematopoiesis of indeterminate potential) is a non-malignant condition characterized by mutational and clonal expansion of hematopoietic stem cells, beginning at middle age. Though CHIP is not considered a disease, it is associated with a higher risk for several diseases including hematological malignancies, cardiovascular diseases and many others. Approximately 20% of the US population have this condition, but most are not aware of it. Understanding the causes and mechanisms of CHIP and how these cells interact with cells of other tissues will provide critical insights into disease development and evolution.

In this panel discussion webinar, speakers will discuss the growing interest in this topic and a few example studies looking at how this condition arises and transforms into a disease. Dr. Ross Levine of Memorial Sloan Kettering Cancer Center (MSKCC) will first discuss the clinical manifestations of clonal hematopoiesis, and mechanisms underlying its development as well as its role in clonal evolution toward leukemia. Then he will invite two other speakers, Dr. Kelly Bolton of Washington University of St. Louis and Dr. Jyoti Nangalia of Wellcome Sanger Institute, for an interactive panel discussion that will offer key insights and diverse perspectives on CHIP as an important risk factor and its clinical impact.


• Ross L. Levine, MD, Memorial Sloan Kettering Cancer Center
• Kelly Bolton, MD, PhD, Washington University of St. Louis
• Jyoti Nangalia, PhD, Wellcome Sanger Institute


Emerge Webinar Series Episode 7

Thursday March 31 at 4 pm (Amsterdam, Berlin, Stockholm)

Defining Proteome Dynamics in the Aging Proteome with Novel Computational Approaches.

Nathan Batisty, NIH, National Institute of Aging

Loss of protein homeostasis is a hallmark of aging and age-related conditions, including neurodegeneration, sarcopenia, and type 2 diabetes. However, alterations in markers of proteostasis machinery do not necessarily reflect rates of protein turnover. Therefore, methods to measure the turnover rates of proteins directly, rather than surrogate measurements of translation and degradation machinery, are critically needed to accurately examine the stability of the proteome during aging and disease processes.
The development of versatile computational tools on widely accessible, open-source platforms is needed to make this approach more user-friendly.
Here, we introduce a new computational tool – TurnoveR – for the accurate calculation of protein turnover rates from mass spectrometry analysis of metabolic labeling experiments in the Skyline software platform.
Using data generated from metabolic labeling of mice with heavy leucine in independent experiments, we demonstrate how this tool enables the calculation of protein turnover rates seamlessly within a Skyline workspace using raw data acquired on multiple mass spectrometric platforms and derive new biological insights into age-related diseases.

Unleasing the Power of Single-Cell Multi-Modal Analysis to Advance Precision Medicine

Thursday March 17 2022

Join Mission Bio and SciLifeLab for an interactive session about the latest advances and applications of single cell technology that simultaneously measures single-nucleotide variation (SNV), copy number variation (CNV), and protein data from the same cells.

We are happy to welcome scientists from the Clinical Genomics Uppsala presenting some of their recent work. In addition we will give a an introduction to Mission Bio’s Tapestri capabilities on SNV, CNV, and protein sequencing at scale, the workflow and its applications in oncology and cell and gene therapy.

Clonal heterogeneity in ALL at diagnosis and during chemotherapy treatment detected by single-cell sequencing

Available on demand

Acute lymphoblastic leukemia (ALL) is an aggressive leukemia that occurs most frequently in children and is characterized by the presence of few chromosomal rearrangements and additional mutations. In this webinar, the second in a “Meet the Authors” series sponsored by Mission Bio, Jan Cools of the VIB Center for Cancer Biology in Leuven, Belgium will discuss the application of single-cell analysis to determine the clonal heterogeneity of the leukemia cells of ALL cases at diagnosis.

Dr. Cools’ lab used single-cell targeted DNA sequencing (Tapestri, Mission Bio) and single-cell RNA-sequencing (10x Genomics) to determine the clonal heterogeneity of the leukemia cells of 20 ALL cases at diagnosis and monitored the clonal evolution during chemotherapy treatment. Specifically, the lab designed a custom ALL panel and obtained accurate single-nucleotide variant and small insertion-deletion mutation calling for 305 amplicons covering 110 genes in about 4400 cells per sample and time point. Bone marrow and/or blood samples from 12 B-cell ALL and eight T-cell ALL patients were analyzed.

Dr. Cools will discuss how single-cell DNA amplicon sequencing is a sensitive assay to detect clonal architecture and evolution of the malignant cells in ALL, and present his findings published in the Blood paper, “Single-cell DNA amplicon sequencing reveals clonal heterogeneity and evolution in T-cell acute lymphoblastic leukemia.”

Watch webinar on demand

Laminar Wash™ enables single-cell multiomics and cell hashing in studies with limited numbers of cells

Professor Mats Bemark, Ph.D.‘s lab at the University of Gothenburg in Sweden studies how the immune system responds to respiratory infections, such as SARS-CoV-2 and influenza. His research focuses on interrogating these interactions at mucosal layers using advanced analytical methods, including flow cytometry and single-cell transcriptomics (scRNA-seq). A key to unlocking the potential of these studies is addressing sample prep challenges, particularly when starting cell numbers are limited.

Topics covered:

  • How to expand scRNA-seq immunology studies with TotalSeq antibodies for multiomics and cell hashing
  • Why proper cell washing is integral to study success
  • The challenges researchers face with a limited number of cells in a given sample
  • How Laminar Wash technology enabled TotalSeq labeling of antigen-specific lymphocytes in a study of mucosal immunology.

Get information about Laminar Wash™ for Single-Cell Sequencing

Flow cytometry standrads


Tuesday 19th October 11 am Eastern (17:00 Copenhagen)

Thursday 21st October 9 am Central Europe live Q&A

Professor Mats Bemark, Ph.D.‘s lab at the University of Gothenburg in Sweden studies how the immune system responds to respiratory infections, such as SARS-CoV-2 and influenza. His research focuses on interrogating these interactions at mucosal layers using advanced analytical methods, including flow cytometry and single-cell transcriptomics (scRNA-seq). A key to unlocking the potential of these studies is addressing sample prep challenges, particularly when starting cell numbers are limited.

Webinar attendees will learn:

  • How to expand scRNA-seq immunology studies with TotalSeq antibodies for multiomics and cell hashing
  • Why proper cell washing is integral to study success
  • The challenges researchers face with a limited number of cells in a given sample
  • How Laminar Wash technology enabled TotalSeq labeling of antigen-specific lymphocytes in a study of mucosal immunology.

Dr. Bemark will be available for a Live Q&A on 21 October at 9AM Central Europe time (15:00 Singapore time). Please use the link below to register for the Live Q&A; we will inform you when the recording is posted to the Curiox website beforehand.

Get information about Laminar Wash™ for Single-Cell Sequencing

Webinar: Navigating through the IVDR Directive and utilising Qnostics within the molecular laboratory

Thursday October 14 at 10 am (CET)

During this webinar our presenters will focus on navigating the IVDR directive and how best to tackle this new initiative. Additionally, our guest speakers will also present a valuable case which focuses specifically on the validation of a direct PCR machine utilising the Qnostics SARS-CoV-2 Analytical Q Panel.

emerge webinar

Emerge Webinar Series Episode 4. Automated and ultra-sensitive workflows for large-scale quantitative phosphoproteomics

Friday October 7, 18:00 CET conference start, 21:05 CET presentation start 

Florian Harking, NNF Center for Protein Research, Olsen Group, University of Copenhagen, Denmark

Phosphorylation is one of the most common post-translational modifications. It plays a key role for intra-cellular signal transduction and is known to be involved in numerous disease mechanisms. Current LC-MS/MS based workflows focusing on identifying phosphorylation are aimed at processing high sample quantities at low throughput. At the same time, a number of clinical sample types, are limited by their sample amount, and less so the total number of samples. To address this discrepancy, significant efforts have been made in the last few years regarding the analysis of low-input sample preparation and automation. Florian will outline some of these efforts while illustrating the benefits of using automated sample preparation for a more streamlined and reproducible workflow.
emerge webinar

Emerge Webinar Series Episode 3 Wednesday July 28 at 17:00 CEST Controls for Quantitative Proteomics (AKA How to Mass Spec Responsibly)

Julia Robbins, Genome Sciences Department, University of Washington, Seattle

The presentation will focus on the application of rigorous controls in quantitative proteomics to actively assess and validate the integrity of experimental methods and quality of data.
Julia attended the University of Washington, graduating with a Bachelor of Science in Biology from 2013-2018. She joined the MacCoss Lab in January of 2018, where her work focuses on the application of quantitative proteomics to investigate neurodegenerative and aging-related disease in mice models and humans, with an emphasis on developing robust and high-throughput methods of sample preparation and data acquisition.

Webinar: A Next Generation Solution to Quality and Efficient Sample Prep for Single-Cell Multiomics Precision Oncology

Tuesday July 27 2021, 5 am – 6 am (CEST)

Single-cell sequencing and multiomics analysis can be powerful tools across the therapy development pipeline, from discovery to diagnostics. The weak signal-to-noise inherent to these methods demands stringent and careful sample preparation, particularly when those samples come from cancer patients.

We are pleased to host Jonathan Scolnick, Ph.D., Founder and Chief Science Officer at Proteona, a precision oncology company based in Singapore. Proteona uses insights from single-cell multiomics measurements to improve cancer patient care. Joining Dr. Scolnick is Chyan Ying Ke, Ph.D., Associate Director of Bioapplications at Curiox. Dr. Ke leads the Curiox R&D team in Singapore.

Webinar attendees will learn:

– How Proteona approaches single-cell multiomics
– Insights gleaned from single-cell analysis for precision oncology
– Specific workflow challenges associated with single-cell multiomics
– How Curiox Laminar Wash™ technology addresses single-cell ‘omics sample prep
– How Curiox enables single-cell multiomics data collection at Proteona

The timing of the live event is tailored for our Asia-Pacific audience. Regardless of your time zone, we encourage all interested attendees to register, leave a question or comment in the field below, and watch the webinar on-demand shortly after its conclusion.

Get information about Laminar Wash™ for Single-Cell Sequencing

celemics and strand

Webinar: Clinical Data Interpretation Service in NGS Workflows

Celemics & Strand Life Sciences Bioinfomratics Solution Service

Celemics provides robust clinical interpretation services through Strand pipeline that combines bioinformatics algorithms, public data from external sources/knowledge databases, visualization interfaces and reporting capabilities, enabling the user to analyze the NGS data and generate clinical report from Celemics panels, for individual samples.
emerge webinar

Emerge Webinar Series Episode 2

Wednesday June 30 at 17:00 CEST

High-throughput spatial-temporal proteomics for the study of phospho-signaling dynamics

Dr. Ana Martinez del Val Novo Nordisk Foundation Center for Protein Research, Denmark

Ana obtained her PhD at the Proteomics Unit in the Spanish National Cancer Research Center, where she was working on quantitative proteomics and phosphoproteomics applied to stem cell biology. In 2019 she joined the lab of Jesper Olsen at the Novo Nordisk Foundation Center for Protein Research in Copenhagen, where she is working on further applications of mass spectrometry-based proteomics to study signaling and disease.

The presentation will focus on the application of high-throughput spatial-temporal proteomics workflows for the study of phospho-signaling dynamics.

Dynamic changes in subcellular localization of signaling proteins is a general concept that eukaryotic cells evolved for eliciting a coordinated response to stimuli. Mass spectrometry-based proteomics in combination with subcellular fractionation can provide comprehensive maps of spatio-temporal regulation of cells, but involves laborious methods that did not include phospho-proteome analysis.

Ana will present a high-throughput workflow, based on sequential cell fractionation, to profile the global proteome and phospho-proteome dynamics across six distinct subcellular fractions. The workflow was benchmarked by studying spatio-temporal EGFR phospho-signaling dynamics in-vitro (HeLa cell cultures) and in-vivo in mouse tissues, and was also employed to assess the spatio-temporal events in response to stress related signaling, such as hyperosmotic shock, revealing cellular relocation of ribosomal proteins.

emerge webinar

Emerge Webinar Series Episode 1

Wednesday June 2 at 16:00 CEST

Dr. Magnus Jakobsson, Lund university

In support of emerging researchers, we are introducing the EMERGE webinar series with a specific focus on highlighting novel methods and solutions that labs are implementing to tackle the challenges associated with applying omics technologies in a routine way.

At ReSyn Biosciences we focus on efficiency and reproducibility to develop novel workflows for biological Mass Spectrometry and Bioseparation.

Dr Jakobsson received a PhD in 2013 from Oslo University with his thesis entitled: “Characterization of novel human protein methyltransferases”, and subsequently joined the the laboratory of Dr Jesper V Olsen at the Novo Nordisk Foundation Centre for Protein Research, Copenhagen University.

Since 2019 Magnus has been based at Lund University, where he is a co-leader of the Proteoforms@LU MS platform, focusing on post translational modifications and protein variants. He is also a research group leader at the department of Immunotechnology, where his research focuses on aspects covering both basic and translational research, including the development of proteomics workflows for analysis of “orphan” PTMs, the quantitative analysis of proteins and their regulatory PTMs for developing new cancer diagnostics.

Randox Acusera

Live Demo of Acusera 24•7 QC Management Software

Tuesday May 25, 1 pm CEST

Following on from our successful webinar, Randox Laboratories are pleased to invite you to an online demo of our Acusera 24•7 QC Management Software.

Throughout the previous webinar, key speaker Margaret Le Roux highlighted the benefits of implementing a quality control software in a laboratory, specifically focusing on the use of sigma metrics, %bias, total error, uncertainty of measurement and other statistical reports.

An automated QC software, such as Acusera 24•7, can help to enhance performance assessment, improve QC strategy and meet regulatory requirements. This demo session aims to provide an insight to our state-of-the-art software package.

molecular webinar

Webinar: The Need for Robust Molecular Quality Assurance in the Wake of the Pandemic

Wednesday May 26, 2 pm CEST

This educational webinar will discuss topics surrounding the importance of third party molecular Quality Control and External Quality Assessment, as well as the clinical impacts of good or poor QC management. Furthermore, Randox Molecular Product Specialists will detail our extensive product ranges of Qnostics (Molecular QC) and QCMD (Molecular EQA).

Acusera 24 7

Webinar on the utilisation of Quality Control software and sigma metrics for the identification of performance issues.

Tuesday May 18, 1 pm CEST

Acusera 24•7 is an automated QC software which helps to enhance performance assessment, improve QC strategy and meet regulatory requirements.

The webinar will specificlaly focus on the use of sigma metrics, %bias, total error, uncertainty of measurement and other statistical reports.

Acusera 24.7 Brochure

Towards strandardizing

Webinar: Towards Standardizing Flow Cytometry Sample Preparation Workflows without Centrifugation

Wednesday May 13 2021

How much further along would you be if your cell and single nuclei sample prep methods were more consistent, less labor-intensive, and retained more precious material? We’ve teamed up with flowcytometryUK and Charles River Laboratories to discuss how Laminar Wash technology can help standardize, optimize, and improve cell and single nuclei suspension prep as the front-end to generating quality flow cytometry data.

Please join us live on Thursday 13 May for a webinar featuring Noma Ngwenya, Application Scientist at Curiox Biosystems, and Christoph Eberle, Ph.D., Principal Scientist II at Charles River, for an informative webinar, featuring:

  • An introduction to Laminar Wash technology in support of flow cytometry
  • Improvements in single nuclei retention and antibody consumption at Cerevance, a clinical-stage CNS therapeutics company
  • Examples of how Charles River is using Laminar Wash to standardize its flow cytometry sample prep

Date: Wednesday May 13 2021
Time: 5:00 pm CEST

webinar insights

Mission Bio Webinar How Do You Gain Insight into Disease Evolution?

Monday April 19 at 9 am CEST

We’re delighted to be hosting a webinar with FIMM, where they tackle some of the most difficult challenges in genomic research by using information from genome sequences to advance the understanding of biology and improve health.

As a pioneer in high-throughput single-cell multi-omic analysis, Mission Bio has developed a platform to genotype and phenotype from the same cell.

During the webinar we will share:

  • General Tapestri Overview including SNV, CNV, and protein capabilities
  • Hematology applications

Date: Monday, April 19, 2021
Time: 9:00 AM CET

Apply siPOOL

Reliable Gene Silencing with Exceptional siRNA Specificity by High Complexity siPOOLs

Thursday April 15 2021 at 3 pm CEST

siRNA pools (siPOOLs) are high complexity pools of 30 optimally-designed siRNAs that were demonstrated to efficiently remove off-target effects and improve reliability of results (Hannus et al., 2014).

siPOOLs are suited for use against coding or long non-coding RNAs, selected isoforms or closely-related genes, and can be applied in combination to study synergistic gene interactions (Welsbie et al., 2017).

siPOOLs are ideal tools in drug discovery for target validation and target identification with custom or ready-made siPOOL libraries.

whole pathogen controls2

Qnostics Quality Controls for Molecular Diagnostic Assays in Infectious Disease

Wednesday March 24 at 10-11 am (CET)

Importance of Independent Third Party Controls

This discussion will provide tangible examples on how to successfully incorporate the use of a variety of different Qnostics controls into laboratories carrying out molecular diagnostic testing:

Test for the dynamic range of a molecular assay using Analytical Q Panels

Test the comparable of Methods vs Robotic systems using Molecular Q Panels

Undertake equipment validations and staff training using Medium Q Controls.

Presented by Dwaine R. Vance BSc (Hons), DIS, PhD.

Mission bio kol seminar series1

Mission Bio Technology and Applications New Single Cell Genomics and Multiomics Service

Wednesday March 10 at 10 am CET

We´re delighted to be hosting a webinar with SciLifeLab clinical Genomics Facility, where they tackle some of the most difficult challenges in genomic research by using information from genome sequences to advance the understanding of biology and improve health.

Cell sample preparation

Centrifuge-Free Automation of Suspension Cell Sample Preparation Webinar

Wednesday February 24 10 am (EST)

Automating cell wash workflows with a centrifuge is challenging and expensive.

Alternatively, Laminar Wash™ technology offers a gentle, reproducible, and high-throughput alternative to preparing suspension cell samples without subjecting them to damaging gravitational forces.

Join us to find out how Laminar Wash:

Overcomes the challenges of automating sample prep

Offers several integration options to streamline your laboratory workflow

Improves cell suspension sample quality for downstream analysis

Malcolm Crook, PhD, Technical Director, Peak Analysis & Automation
Ann Wang, Field Application Specialist, Curiox Biosystems