NGS, PCR & qPCR
Read more about Direct PCR of Crude Samples without Template Purification, highQu PCR & qPCR Reagents Performance Excellence etc.
Read more about Direct PCR of Crude Samples without Template Purification, highQu PCR & qPCR Reagents Performance Excellence etc.
Magnetic bead based depletion of fragments <10 kb
Long read sequencing library cleanup & size selection
High and ultra-high molecular weight extraction kit for long read sequencing.
Biolegio offers a range of products, spanning from highly modified and specialised oligos, to standard DNA oligo synthesis.
Applications covered are sequencing, NGS, PCR, Realtime-PCR, SNP detection, genotyping, gene expression and mutation detection.
Universal, Efficient Ribosomal RNA Depletion for Any Species
The riboPOOL kits are availabble for a range of indivisual species, as well as combined species from similar or different species types.
The kit now also includes a RNA cleanup module consisting well validated RNA cleanup beads.
Economy:
The efficient removal of rRNA provides huge cost savings in RNA sequencing. The riboPOOLs are complex probe sets that binds with high specificity to the ribosomal RNA of your samples.
Flexibility:
riboPOOL kits are available as single-species riboPOOLs, Pan-riboPOOLs for similar species types or Combined riboPOOLs for non-related species.
For special applications, riboPOOLs are also available for abundant mRNA removal, FFPE/degraded RNA samples and a special riboPOOL for ribosome profiling, ribo-Seq.
Simplicity:
Easy to use system with integrated touch screen and intuitive, protocol-based software.
Built-in Wi-Fi, ethernet and USB allow for remote control and make transferring data easy.
Sensitivity:
Efficient illumination and detection of each well provides exceptional specificity and precision.
Flexibility:
Up to 6 fluorescent channels allow for flexible dye
usage and broad application.
Fast PCR protocols applicable. Simultaneous scanning of 16 wells provides faster scanning speed compared to individual well scanning technology.
Reproducibility:
The robust platform provides optics and thermal block designed to deliver the same uniformity and reproducibility over at least 1000 qPCR experiments, giving you confidence in the reliability of the instrument.
A rRNA depletion kit for Caenorhabditis elegans is now available for RNA-Seq.
riboPOOLs are biotinylated single stranded DNA-oligos specifically designed to bind to the species specific rRNA. Streptavidin beads are then used to pull out the rRNA.
Depending on the application you are interested in, we offer different oligo length and compositions:
Standard RNA-Seq riboPOOLs of the rRNA sequences
28S, 18S, 5.8S, 5S and mito 16S & 12S
FFPE samples /ribosome profiling riboPOOLs
offer full length overlapping oligos which cover
the complete rRNA of C.elegans.
Compact & Fully Automated Magnetic Bead Based Extraction
The new NAP16 system provides automation and ease-of-use in magnetic bead based extraction protocols.
4X 1Step RT qPCR Probe Kits are designed for a sensitive detection of specific RNAs, including virus RNA, in diluted high volume samples. They combine a robust 4X qPCR mix with a 20X
blend of thermostable Reverse Transcriptase and RNase
Inhibitor.
Isothermal amplification of nucleic acids based on performance of such strand displacement polymerases as phi29 or Bst is a powerful and simple method to detect and amplify low abundance DNA and RNA targets within a short period of time and without the thermal cycler.
The Azure Biosystems™ Cielo™ 3 and 6 real-time PCR Systems are designed to offer smart protocols, high quality data, robust durability and an intuitive user experience.
Our novel fiber optic detection system allows for enhanced sensitivity and speed. 3 or 6 standard channels let you analyze a variety of commercially available dyes, already in your workflow. Onboard software operated by a 10.2″ touchscreen minimizes footprint and saves you bench space.
Easily connect to your Cielo via Wi-Fi, ethernet, USB or LAN. Get notified after each completed run and even remotely access protocols and files.
Application
SampleIN™ Direct PCR Kit
Rapid 15 min DNA extraction using DPK Lysis and Protease Buffers in a single tube generates PCR template extract which is further amplified under fast cycling conditions with a hot-start Taq master mix that includes red dye for direct gel loading.
Allows direct fast and efficient PCR from various sample types: blood, mouse tail/ear, tissue, buccal swabs, hair follicle, plants.
Contact us about your needs to improve your PCR reactions.
Choose from a range of easy-to-use, fast, highly sensitive and robust polymerases.
Highly accurate DNA polymerase for increased sensitivity and great multiplex results.
Fast, high yield PCR with the fidelity 100x higher than Taq.
High thermostability for better denaturation of GC rich templates.
Up to 20 kb long PCR even from complex templates.
Increased processivity for faster amplification and higher yield.
Available as hotstart and non-hotstart.
Get polymerase plus optimised ALLin™ Buffer, or ready-made mastermixes, with or without red dye.
highQu PCR reagents are highly efficient polymerases, available in a flexible range of products depending on application.
Great for challenging templates or crude samples.
Developed for a universal use, fast protocols and high user convenience.
The highQu 1Step RT-qPCR kit is successfully used in Covid-19 testing.
With an advanced RNAse inhibitor, an efficient Reverse Transcriptase and a robust qPCR polymerase, this kit is a highly efficient tool for low copy number detection.
<5 copies detected
The 4X mastermix allows higher sample volumes (10 ul sample in a 20 ul reaction) for low viral load detection.
Compatible with all instrument platforms.
Efficient RNAse inhibition even at higher temperatures up to 60°C.
RNA protection from hydrolysis by RNases A, B and C.
Economical and stable for weeks at ambient temperature.
Robust, works well at 5.5 to 9.0 pH, with 0.5 – 1 mM DTT.
Successfully tested in Sars-CoV-2 detection workflows.
Available in different OEM formats
highQu PCR reagents are highly efficient polymerases.
The products range is flexible depending on application. Great for challenging templates or crude samples.
Developed for a universal use, fast protocols and high user convenience.
Order here
Check our video with tips and tricks for your successful PCR testing.
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