ALLin™ Hot Start Taq Mastermix, 2X
1000 r of 50 µl
Applications
Hot-start PCR up to 6 kb with a direct gel loading option
Crude sample PCR
Low copy target detection
Amplification of complex (GC/AT rich) templates
Fast PCR
TA cloning
Multiplex hot-start PCR
Benefits
Outperforming sensitivity & specificity – low copy number target detection and no background
Higher yields under standard and fast cycling
Increased sensitivity and success in amplification of longer templates (6 kb), robust amplification of GC rich templates
Premixed with the red dye and density reagents for direct loading on the gels after the PCR
highQu ALLin™ Hot Start Taq DNA Polymerase is the superior sensitive enzyme. The activity at room temperature is blocked by small molecular inhibitor. Enzyme becomes active only after heating what allows for highly specific and extremely sensitive amplification, no primer dimer formation and no background.
In combination with the optimized ALLin™ buffer enzyme provides higher success rates in demanding PCR applications like amplification of crude, complex or longer templates and fast cycling.
ALLin™ Hot Start Taq DNA Polymerase has the same PCR accuracy like Taq DNA Polymerase, 4.5 x 104 (nucleotides incorporated before the error occurs) and produces A-tailed products suitable for ligating into TA cloning vectors.
The convenience of ALLin™ Hot Start Taq DNA Polymerase is maximized by the use of 2X Red colored Mastermix providing the additional advantage of reduced pipetting and minimized errors. The mastermix is even supplied with PCR Water, and the only thing to add is the template with primers.
ALLin™ HS Red Taq Mastermix, 2X is premixed with red dye and density reagents for direct loading on the gels after the PCR.
In a 2% agarose TAE gel the dye migrates with~350 bp DNA, in 1% agarose TAE gel with ~ 600 bp DNA fragments.
ALLin™ HS Red Taq Mastermix, 2X is also a key component in highQu SampleIN™ Direct PCR Kit (DPK0101/5), ensuring outstanding PCR results with crude samples.
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